Simo Ingride*;
Nganou Blaise;
- 5
G. Sonfacka, C. F. Tchindab,c, G. T. M. Bitchagnoa,, I. K. Simoa*, B. K. Nganoua, İ. Çelike, M. Tenea, V. P. Bengc, V. Kueteb, P. Tanea
a,bUniversity of Dschang,
cUniversity of Yaounde I
Background: Resistance of bacteria to antibiotics one of the biggest problem on the world. The present word was designated to evaluate the antibacterial activity of saponins as well as their antibiotic-potentiating effects towards eleven Gram-negative multidrug resistant (MDR) bacteria.
Methods: The methanol extract obtained by maceration was partitioned into ethylacetate and n-butanol fractions respectively. Column chromatography followed by purification of different fractions was applied to isolate two saponins from the n-butanol fraction and their chemical structures were determined using spectroscopic techniques. The antibacterial activities of the samples (determination of Minimal Inhibitory « MIC » and Minimal Bactericidal Concentration « MBC ») were determined by the modified rapid p-iodonitrotetrazoliumchloride (INT) colorimetric assay, as well as those of antibiotics in association with the compounds.
Results: The phytochemicals isolated were adianthifolioside GS1 (1) and 3-O-{β-D-glucopyranosyl(1→2)-[α-L-arabinopyranosyl(1→6)]-β-D-glucopyranosyl}oleanolic acid (2). The fractions and isolated compounds were tested against eleven Gram-negative bacteria including multidrug resistant strains. Ethylacetate fraction exhibited selective inhibitory effects whilst all tested bacteria were inhibited by n-butanol fraction in MIC ranges of 8 to 1024 μg/mL. Compound (2) inhibited the growth of 6/11 (54,5%) tested bacteria, with the MICs ranging from 32 to 128 μg/Ml. Compound (1) revealed considerable inhibition against all the studied bacteria with MIC values ranging from 16 to 128g/mL.
Conclusion: The present study provides information on the possible use of Albizia adianthifolia and its constituents in the control of Gram-negative infections including MDR phenotypes.
Keywords: Albizia adianthifolia, Antibiotic modifying activity, Mimosaceae, Phytochemicals, Multi-drug resistance,
