Anthelmintic activity of Combretum mucronatum against four gastrointestinal nematodes
Belga François Ngnodandi1, Jato Jonathan2, Waindok Patrick3, Spiegler Verena4, Hensel Andreas4, Strube Christina3, Ndjonka Dieudonné1*.
Background: The increasing prevalence of anthelmintic-resistant strains of gastrointestinal nematodes and high cost of conventional anthelmintic has incited researchers to find an alternative to the synthetic anthelmintic drugs. Medicinal plants rich in condensed tannins showed the potential anthelmintic against gastrointestinal nematodes and the mutant strains of Caenorhabditis elegans. The aim of this study is to evaluate the potential anthelmintic of Combretum mucronatum leaves on gastrointestinal nematodes of dogs and pigs. Methods: Hydro-ethanolic extract of Combretum mucronatum leaves was prepared, analysed and standardized by UPLC. TLC was used for qualitative phytochemical compounds analysis. Anthelmintic activity was assessed with increase concentrations on the third stages of the A. caninum, A. suum, T. canis and T. suis to determine the IC50. Results: TLC revealed proanthocyanidins and flavonoids. After characterization, procyanidin C1, epicatechin, catechin, isoorientin, vitexin, procyanidin b5 were identified. The extract contains 1.03 and 16.35 % of epicatechin and catechin respectively. The higher activity was revealed against T. suis and A. suum with IC50 values of 4.28 ± 2.36 and 5.75 ± 4.79 µg/mL respectively. IC50 of the extract on T. canis and A. caninum was 150 ± 28.36 and 34 ± 14.9 µg/mL respectively. Migration inhibition assays were dose-dependent. Conclusion: C. mucronatum leaves extract showed an anthelmintic efficient against nematode parasites of dogs and pigs. This plant contains bioactive compounds responsible for anthelmintic efficacy.
Keywords: Gastrointestinal nematodes, Combretum mucronatum, Anthelmintic, Eipcatechin, Catechin.
Activité Anthelminthique de Combretum mucronatum sur quatre nématodes gastro-intestinaux